RNA-seq identification of herpes simplex virus type 1 transcription in Neuro-2a cells overexpressing FOXK1 and FOXF1

We used inducible Neuro-2a cell lines with stable expression of FOXK1 and FOXF1. The cells were induced to express FOX protein under doxycycline for 36 hours and then infected with HSV-1 for five hours. The cells were collected and cleaved, and total RNA was extracted for RNAseq analysis Through RNAseq analysis, we mainly focused on the changes in the number of viral reads under the condition of FOX protein overexpression. P stands for wild-type Neuro-2a cells, K stands for Neuro-2a cells that overexpress FOXK1, and F stands for Neuro-2a cells that overexpress FOXF1.