Whole genome methylation analysis in blood and in specific cell blood subtypes in Parkinson’s disease.

we analysed the whole epigenomic profile of different blood subtypes, neutrophiles, monocytes, lymphocytes B and T, from 5 de novo and drug-naïve PD patients and 5 age and gender matched healthy control. 1st experiment: DNA from Lymphocytes B and T, monocytes and neutrophils of 5 controls and 5 incidental parkinson's disease patients (PD) were isolated using Milteinyi purification columns. Infinium Human Methylation 850K BeadChip Kit (Illumina) was used to analyse the methylation pattern. 2nd experiment:Whole DNA from blood of 15 PD and 15 controls were obtained by standart methods and analysed by using Infinium Human Methylation 850K BeadChip Kit (Illumina).The EZ DNA Methylation Kit (Zymo Research) was used for bisulfite conversion of 1,000 ng genomic DNA following manufacturer instructions. Bisulfite‐converted DNA was hybridized onto the Infinium Human Methylation 850K BeadChip Kit (Illumina).