Comparison of extravillous trophoblast cells derived from human embryonic stem cells and from first trimester human placentas

The goal of the present study was to characterize HLA-G-positive cells derived from human embryonic stem cells treated with BMP4 for five days and compare them to HLA-G positive cells derived from human first trimester placentas Human embryonic stem cells were cultured with BMP4 in the absence of FGF2 for 5 days. They were then dispersed and separated into HLA-G+ and HLA-G-, TACSTD2 positive cells by using immunomagnetic bead separation. First trimester human placental samples from 8-12 weeks gestation were enzymatically separated into single cells, and then also separated into HLA-G+ and HLA-G-/TACSTD2+ cell populations