CTCF (CCCTC-binding factor) binding patterns in the human genome following exposures to Clostridioides difficile TcdB.

This experiment uses a CUT&RUN approach to determine if the CCCTC-binding factor (CTCF) is altered in its ability to bind the human genome following exposure to TcdB. TcdB is a bacterial toxin and primary virulence factor produced by the enteric pathogen C. difficile. CTCF is a DNA binding protein that regulates gene transcription in the host. Overall design: HeLa cells exposed to 1 ng/ml of TcdB2 for 24 h were compared to untreated controls. Following treatment, CUT&RUN was used to map genomic sites binding CTCF and H3K4me3.