A Human Brain Map of Mitochondrial Respiratory Capacity and Diversity

Supplementary Video 1 | Human brain slab voxelization using a computer numerical control (CNC) cutter. Milling was done over ~5h at -25°C (freezer room) with routing of the drill bit controlled by a computer in the next room (+4°C).

Supplementary File 1 | Extended discussion of the loadings for MRI-based metrics and mitochondria biology from Extended Table 1.

Source Data 1 | Analysis of snRNAseq data. Detailed markdown of the single nucleus RNA sequencing (snRNAseq) analysis, including the code for data pre-processing, visualization, cluster identification and mitochondrial analysis. This file also shows additional figures supporting the analysis. An HTML file also available at http://humanmitobrainmap.bcblab.com.

Source Data 2 | A complete dataset of mitochondrial parameters (CI-IV activities, MitoD, TRC and MRC) and their correlations with MRI and PET metrics. An Excel spreadsheet with MRI and PET values for each voxel.

Source Data 3 | Software for the voxelization of the tissue. Igor Pro procedure file for 1) defining the parameters to clean and partition a frozen brain slab, 2) generating G-codes used to control the CNC router, and 3) randomizing samples and assigning them to specific assay plates.

Source Data 4 | Software for the voxelization of the tissue. Igor Pro procedure file for 1) derandomizing the samples after the assays are complete, and 2) plotting the assay data on the topological map of the brain.

Source Data 5 | Morphing the image of the brain onto MNI space coordinates. Python code used for the conversion of the image of the brain slab to Neuroimaging Informatics Technology Initiative (NIfTI) format.