Morphological defects of somites in zebrafish embryos injected with morpholinos against CARM1 or PRMT5.

14 ss: embryos were injected at the one cell stage with 6 ng of morpholinos against CARM1 (MoC1), PRMT5 (MoP5) or control morpholinos (MoCt). Rescue experiments were performed by co-injecting MoC1 or MoP5 with the mRNA coding for CARM1 (MoC1+C1) or PRMT5 (MoP5+P5), respectively.24 hpf: Morpholino doses used for 24 hpf phenotype analysis were the following: 3 ng (a), 6 ng (b) and 12 ng (c). Percentages of embryos exhibiting the various phenotypes at 14 ss and 24 hpf are shown.*Round, smaller somites;#Flat, longer somites;**Shorter axis.