Molecular mechanisms of NEXT complexes regulating H3K27me3 levels and affecting tumor progression (ChIP-Seq)

H3K27me3 is an important post-translational modification of histones and is responsible for establishing and maintaining transcriptional repression in heterochromatin regions. The PRC2 complex is the only complex identified with catalytic activity of H3K27me3, and its functional activity is subjected to a wide range of regulation, in which non-coding RNAs play a key regulatory role. The NEXT complex is an RNA exosome-targeting complex localized in the nucleoplasm and is responsible for the degradation of a large number of non-coding RNAs in the nucleus. However, the functional relationship between the NEXT complex and the PRC2 complex or the regulation of H3K27me3 levels has not been reported. In this study, we found that the NEXT complex promotes the recruitment of the PRC2 complex, especially EZH2, to chromatin by degrading nascent RNAs containing the G4 structure, which in turn promotes the level of H3K27me3 modification and cancer progression. Overall design: Chromatin immunoprecipitation DNA sequencing for EZH2 as well as the histone modification H3K27me3 in ACHN cells.