Additional file 2 of Identification of a universal antigen epitope of influenza A virus using peptide microarray

Additional file 2:. Microarray data. Synthetic peptides were sampled onto iPDMS (modified silica gel film) to form the chip. For the binding activity assay, sera were diluted 1:50 (v/v) with serum dilution buffer. Signal-to-noise ratio (SNR, SNR = signal strength–background intensity)/background intensity) was determined by GenePix Pro 6.0 software. H5–01: serum against A/Mallard/Huadong/S/2005; H5–02: serum against A/Duck/Huadong/wx1205/2016; H5–03: serum against A/Chicken/Huadong/yz1111/2016; H5–04: serum against 2.3.4.4d vaccine strain; H5–05: serum against 2.3.2.1d vaccine strain; H7–01: serum against A/Chicken/Jiangsu/W1–8/15; H7–02: serum against A/Chicken/Huadong/JD/17; H9–01: serum against A/Chicken/Shanghai/F/98; H9–02: serum against A/Chicken/Taixing/10/2010. Each reaction was repeated for 3 times, and SNRs were expressed as means ± standard deviation.