Effect of RAGE on macrophage transcriptome in regression of diabetic atherosclerosis. Effect of RAGE on macrophage transcriptome in regression of diabetic atherosclerosis

Ldlr null (Ager+/+ Ldlr-/-) diabetic mice were raised on a Western diet. Their aortas were transplanted to diabetic mice rasied on standard chow, which were either WT (Ager+/+ Ldlr+/+) or Ager null (Ager-/- Ldlr+/+). The gene expression profile of either the donor (CD45.2) or recipient (CD45.1) sorted macrophages from both genotypes was studied. Overall design: Ldlr-/- Donor: Macrophages from the aortas of Ldlr null CD45.2 diabetic mice fed a Western Diet, and not transplanted (n=4) WT Recipient: WT recipient macrophages, from the transplant: Aortas of Ldlr null CD45.2 diabetic mice fed a Western Diet, and transplanted into standard chow-fed WT CD45.1 diabetic mice (n=4). Ldlr-/- Donor → WT: Ldlr null donor macrophages, from the transplant: Aortas of Ldlr null CD45.2 diabetic mice fed a Western Diet, and transplanted into standard chow-fed WT CD45.1 diabetic mice (n=4). Ager-/- Recipient: Ager null recipient macrophages, from the transplant: Aortas of Ldlr null CD45.2 diabetic mice fed a Western Diet, and transplanted into standart chow-fed Ager null CD45.1 diabetic mice (n=6). Ldlr-/- Donor → Ager-/-: Ldlr null donor macrophages, from the transplant: Aortas of Ldlr null CD45.2 diabetic mice fed a Western Diet, and transplanted into standard chow-fed Ager null CD45.1 diabetic mice (n=4).