Statistical analyses of fly size variation at the start vs the end of positive size selection.

Repeated measures ANOVA statistics for the first and last generations that experienced directional selection for larger size, comparing hypoxic-reared (10 kPa, top) or hyperoxic-reared flies (40 kPa, bottom) to the control or normoxic-reared flies (21 kPa). Significant p values are boldfaced. In all cases, hypoxic-reared flies were significantly smaller than normoxic-reared flies, and responded differently than normoxic-reared flies. 10 kPa flies had a lesser increase in mass with size selection, indicated by significant aPO2×Generation terms. (F = F-ratio; DF = degrees of freedom).