FAIRsharing record for: Minimum Information about a Single-Cell Experiment

This FAIRsharing record describes: minSCe is a minimum set of single-cell metadata categories and a checklist of information that can be used to describe a single-cell assay in sufficient detail to enable the analysis of transcriptomic data. These guidelines are derived from work on building and adapting community resources to archive and add value to datasets, such as the Expression Atlas, the HCA-Data Coordination Platform and the CIRM Stem Cell Hub.

A single-cell sequencing experiment is described by adding custom fields to the five main components of the MIAME and MINSEQE experiment model. Each component is equivalent to a main experimental step. Information is captured describing each component and linking it to the relevant protocols. For each field, it is recommended to use terms from a suitable ontology (e.g. NCBI taxonomy for species) or controlled vocabulary to prevent ambiguity. More information is available at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302581/.