Additional file 1. Primers used for bisulfite-based PCR with subsequent SNuPE and local deep bisulfite sequencing (MiSeq). For MiSeq universal TruSeq adaptor sequences were attached to the specific se
Miniaturized biological assays using microfluidics have the potential to enhance assay sensitivity, reduce reagent consumption, and increase throughput. However, challenges to miniaturization incl
We performed UPLC-MS/MS to investigate global 5mC and 5hmC levels in sixteen pairs of HCC and non-HCC samples and two HCC cell lines (97L and LM6 cells). We found that 5mC and 5hmC were frequently dec
