TMT-based quantitative proteomic analysis of spheroid cells of endometrial cancer possessing cancer stem cell properties

RNA-seq of HK2-siRNA transfected to Ishikawa and HEC1A cells to investigate the molecular mechanism of HK2 in endometrial cancer. Total RNA extraction in four groups (Ishikawa-NC V.S Ishikawa-HK2-siRNA, HEC1A-NC V.S HEC1A-HK2-siRNA) was used by Trizol for library preparation, and the sequencing platform was Illumina sequencing, which was completed in Sangon Biotech (Shanghai, China). DESeq2 software (version 1.16.1) was applied to investigte differential expressions between two comparative combinations (Ishikawa-NC V.S. Ishikawa-HK2-siRNA, HEC1A-NC V.S. HEC1A-HK2-siRNA).