Universal_amplification__next_generation_sequencing_and_assembly_of_HIV_1_genomes

Large-scale sequencing of RNA viruses such as HIV-1 is challenging due to the high genetic diversity and the need to generate substantial amounts of DNA. We report a universal method for amplification, next-generation sequencing and unbiased De Novo assembly of HIV-1 genomes that is independent of knowledge about sequence or subtype. We apply our approach to investigate HIV-1 diversity and to identify drug resistance mutations present at low frequencies.