Integrative transcriptomic analysis uncovers the miRNA-centric regulation of Japanese encephalitis virus infection in porcine trophoblast cells [RNA-Seq]

To characterize porcine placental miRNAs, we performed small RNA-seq experiments using porcine placental tissues and the porcine placental trophoblast cell line pTr. To investigate the intracellular responses of pTr cells following JEV infection, RNA-seq and small RNA-seq experiments were conducted on pTr cells with or without JEV infection. Overall design: We collected placental samples from first - parity naturally delivered Duroc gilts, pTr cell samples under normal culture conditions, and pTr cell samples at 24 and 48 hours post - infection with JEV as well as non - infected samples. Two replicates per sample were used for RNA-seq and small RNA-seq experiments.