Additional file 8 of DIMPLE: deep insertion, deletion, and missense mutation libraries for exploring protein variation in evolution, disease, and biology

Additional file 8: Table S5. Baseline Kir2.1 library errors. Error counts within the sequencing data. Because Illumina sequencing platforms have baseline error rates to a degree this is a combined error between sequencing and OLS DNA synthesis. Errors are broken up between deletions and insertions broken up across multiple length classes, point mutations that would result in synonymous and missense mutations, other which includes multiple mutations within a sequence which is a common within OLS subpool that many oligos with error will have multiple. Also included are the sum total and the number read counts of expected designed variants within the library. From this we can calculate that about 80% of our library are designed variants while 20% are not.