The Tumor Microenvironment of Laryngeal Squamous Cell Carcinoma Revealed by Single-Cell Transcriptomics

Laryngeal squamous cell carcinoma (LSCC) is a malignant tumor with a high possibility of metastasis. The tumor microenvironment (TME) is well known to play an important role in LSCC metastasis. To gain insights into the TME of LSCC, we performed single-cell RNA-seq (scRNA-seq) using LSCC with lymph node metastases. Overall design: We collected 10 fresh tissues immediately after surgery, including carcinoma in situ (T), normal laryngeal mucosal epithelia (N), margins of cancer (R), and lymph nodes (L). Single-cell suspension was prepared immediately and then subjected to 3' droplet- based single-cell sequencing (10×Genomics)