Mouse liver fxr chip-seq II
收藏NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE259266
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资源简介：
We analyzed genome-wide FXR binding in liver of mice treated with GW4064 by ChIP-seq Freshly liver samples was fixed in 1% formaldehyde for Cross-Linking of DNA and Protein. Liver was sonicated to fragment chromatin DNA.Chromatin was immunoprecipitated overnight at 4 °C using FXR antibody (Cell Signaling Technology, 72105) .The sequencing libraries of input and IP were generated by NEBNext®UltraTM DNA Library Prep Kit for Illumina.The library products corresponding to 1100-500 bps were enriched, quantified and finally sequenced on Novaseq6000 sequencer (Illumina) with PE150 model.
创建时间：
2024-11-03
