CMS-S maize RNA-seq

The S-type cytoplasmic male sterility (CMS-S) is the largest group of maize CMS with a wide range of cytoplasmic sources.S-Mo17-rf3rf3 is a S type CMS line with the nuclear background of maize inbred N-Mo17-rf3rf3, and S-Mo17-Rf3Rf3 is a near isogenic line of S-Mo17-rf3rf3 harboring the main restoration gene, Rf3. The maize inbred W2 is an inbred with another restoration gene, Rf10.To map and clone the restorer genes, Rf3 and Rf10,the BC1[(S-Mo17-rf3rf3 x S-Mo17-Rf3Rf3) x N-Mo17-rf3rf3] and BC2{[(S-Mo17-rf3rf3 x W2) x N-Mo17-rf3rf3] x N-Mo17-rf3rf3} populations were generated. BSR-seq was performed to map the Rf3 gene with the RNAs of two extreme plant pools from BC1 population and to map Rf10 with BC2 population.