D. simulans Lhr interacts more strongly with Hmr than D. melanogaster Lhr.

Transgenic Lhr orthologs were tested for interaction with two Hmr alleles: a null and a hypomorph, specified in the table as Df(1)Hmr− and Hmr1 respectively. D. melanogaster female parent genotypes were: null mutation, y w Df(1)Hmr− v/FM6; φ{transgene, w+}/+ and hypomorph, w Hmr1 v; φ{transgene, w+}/+. Transgenes are denoted as φ{} in the table. Each genotype was mated separately to males from D. simulans v or D. mauritiana Iso105. Hybrid male progeny that inherit the transgene are orange eyed, while the sibling brothers are white eyed. The FET is comparing the relative viability of hybrid sons inheriting the D. melanogaster Lhr transgene vs the relative viability of sons inheriting the D. simulans Lhr transgene in parallel crosses. (n.d. = not determined;*, p≤0.05;**, p≤0.01;***, p≤0.001). RT = room temperature.