Hedgehog signaling affects platelet-derived growth factor signaling mediated alveolar septum formation during postnatal lung development [bulk RNA-seq]

HH inhibition reproduces the lung phenotype of PDGF pathway knockout lungs and results in profound effects on differentiation and gene expression of Pdgfra-expressing alveolar mesenchymal cells, in particular myofibroblasts. Single cell lung suspensions of PgfraEGFP/+ reporter lungs (P8) from mice treated with anti-HH antibody 5E1 or control IgG at E18.5 were sorted based on endogenous EGFP fluorescence and were prepared for bulk RNA-seq.