Improved Scalability, Sensitivity, and Interpretability of Pathogen Detection, Including SARS-CoV-2, in Wastewater using High-Throughput, Highly Multiplexed Digital Array PCR Technology

Background: The goal of this project was to successfully navigate limitations toward development of an advanced digital array PCR system as part of the development of a public health warning system that was not dependent on clinical testing and could rapidly address novel pathogen thrust in the future.

Materials/Methods: Raw influent wastewater samples used in this study were collected as part of the routine monitoring conducted by the North Carolina National Wastewater Sewage Surveillance System.

Outcome/Impact: This study provided valuable practical information for minimizing decay and/or loss of SARS CoV-2 in wastewater influent while adhering to safety procedures, promoting efficient laboratory workflows, and accounting for sources of variability.