Off-target responses in the HeLa proteome subsequent to transient plasmid-mediated transfection.

Data from ProteomeXchange, PXD ID: PXD001278. File: 140508_SILAC_transfect_HeavyReagent_LightNormal_2.mzml. Published as part of Biochim Biophys Acta. 2014 Nov 5;1854(1):84-9 . From the Abstract: {{i}} Here we have used a SILAC-based proteomic approach to study differentially expressed proteins after transfection of HeLa cells with ECFP vector using a commonly employed non-liposome based transfection reagent, Fugene HD. Whereas the transfection reagent itself mediated minimal effects upon protein expression, 11 proteins were found to be significantly upregulated after transfection, all of which were associated with an interferon type I/II response. The upregulated proteins might potentially inflict major cellular processes such as RNA splicing, chromatin remodeling, post-translational protein modification and cell cycle control. {{/i}}