Lipidomics Methods Lipid extraction: HEK293T cells were seeded in 6-well plates, grown to 70% confluency and treated with bumped fatty acid probes (15 µM) for 4 h. Cells were dislodged i
Scatter plot of PCA including the proportions of all identified fatty acids in the non-fractionated extract and 4 lipid fractions of serum and follicular fluid
Direct infusion mass spectrometry serum phosphatidylcholine acyl-alkyls (PC ae) (μM) from 14 lean (L-MAINT) and 16 obese (O-MAINT) cats during weight maintenance and after 10-week energy restriction (
Lipid, an important organic component in milk, has health benefits for newborns. Profiles of milk lipid in multi-species from multi-level haven’t been accessible, therefore, we established a lipid sub