mRNA and lncRNA expression analysis of irradiated and nonirradiated human HaCaT cells

This study aims to compare mRNA expression between radiated and non-radiated human keratinocyte HaCaT cells by microarray analysis. Human keratinocyte HaCaT cells were divided into two groups, each group has three repeats. The cells were irradiated with a single dose of 0 or 20Gy of X-ray irradiation. 48 hours post radiation, cells from 0 or 20 Gy groups were collected and subjected to microarray analysis. mRNA and lncRNA profilings of each group were analyzed by microarray. This study aims to examine mRNA and lncRNA expression spectrum of irradiated and non-irradiated human keratinocyte HaCaT cells. Human keratinocyte HaCaT cells were divided into two groups, each group has three repeats. The cells were irradiated with a single dose of 0 or 20Gy of X-ray irradiation. 48 hours post radiation, the 0 and 20 Gy irradiated cells were collected by Trizol and subjected to microarray analysis.