Identification of YTHDF1 targeted m6A modified transcripts in lung cancer cells

In addition to perform the m6A-seq in A549 cells, we sequenced RNA obtained from the immuno-purified complex of YTHDF1 (RIP-seq) to reveal YTHDF1 bound mRNAs, 3,676 genes were shared (m6A-seq+RIP-seq) as high-confident targets of YTHDF1 , which were mapped to cell cycle and tumor (including lung cancer) related signaling pathways in the KEGG pathway database Overall design: The transcriptome, m6A modified transcripts, and YTHDF1 binding transcripts were identified.