Cost and runtime parameters of CloVR pipeline runs on example datasets.

1Rounded to the next full dollar.2VM instances are linked together as a cluster for parallel processing on the cloud. The number of instances in a cluster can change during pipeline execution. The master instance is included.3The standard CloVR-Metagenomics pipeline refers to the gene prediction-independent protocol.4For the CloVR-Metagenomics pipeline sequence reads are clustered, representative reads for each cluster searched against the reference database, and matches of the representative reads assigned back to all reads from the cluster, resulting in a slightly larger number of overall matches than for the comparable CloVR-Search pipeline.5Scaffold or contig N50 is a weighted median statistic such that 50% of the entire assembly is contained in scaffolds or contigs equal to or larger than this value.6CDS, coding sequences.