Antioxidant program of islet-resident macrophages preserves ß cell physiology

Macrophages residing within mouse pancreatic islets display an activated phenotype at steady state, yet the molecular signatures underlying their polarization remain unclear. To investigate islet macrophage heterogeneity under physiological conditions, we isolated islet-resident immune cells from chow diet-fed adult male mice and performed single-cell RNA sequencing. Overall design: Islets were isolated and pooled from 6–8 mice, dissociated into single cells, and CD45+ immune cells were FACS-sorted. These cells were then subjected to single-cell RNA sequencing using the 10X Genomics platform.