spatiotemporal single-cell transcriptomics in mouse and human to investigate TAM evolution during GBM progression

Glioblastoma (GBM) is the most common and malignant primary brain tumor. Although immunotherapy has shown promise in certain cancer types, it has not been effective against GBM, largely due to its highly immunosuppressive tumor microenvironment (TMEs), which is rich in tumor-associated macrophages/microglia (TAMs). TAMs in late-stage GBM contribute to T-cell exhaustion and worsen prognosis, but the role of TAMs in earlier stages of tumor development is unclear. By employing genetically engineered mouse models and human samples, we used spatiotemporal single-cell transcriptomics to investigate TAM evolution during GBM progression. Overall design: 1,Single immune cell sequencing of spatial sampling (paired Periphery, Border and Core) in 5 implanted QPP#7 mouse GBMs, and in 4 human GBM masses. 2,Single immune cell sequencing of longitudinal sampling of 6 spontaneous QPP Mouse GBM 3,Single immune cell sequencing of Vehicle- or KD3010-treated GL261 mouse GBMs. 1,Single immune cell (CD45+) sequencing of spatial sampling (paired Periphery, Border and Core) in 3 implanted QPP#7 mouse GBMs, and in 3 human GBM masses (Emory Cohort). 2,Single immune cell sequencing of Mg-TAMs and Mo-TAMs