Determination of Shavenbaby target genes in Intestinal Stem Cells (ISCs) [RNAseq_ISC]

Shavenbaby (Svb) transcription factor is involved in the differenciation of epidermal cells and the homeostasis of intestinal stem cells (ISCs). Svb is produced as a long repressor protein called SvbRepressor (SvbREP). In presence of Pri peptides SvbREP is processed into a shorter activator form (SvbACT) through partial proteasomal degradation of the REP domain. In ISCs, Pri triggers SvbREP to ACT maturation, while SvbREP form accumulates in differentiated enterocytes. The absence of Svb promote the apoptosis of the intestinal stem cells. In ISC, the ectopic expression of SvbREP form promote the differenciation and the SvbACT form overexpression promotes hyperplasia. (Al Hayel, et al 2020) Based on these functional analyses, Svb appears to control ISC survival and behavior, balancing proliferation and differentiation through a molecular switch between its 2 antagonistic isoforms. In this study, we determine the Svb target genes in ISCs. To this aim, we analyze the transcriptomes of ISCs upon modulation of Svb function. The samples correspond to control (ISC Ctrl), Svb-knock down ISCs (RNAiSvb), SvbACT constitutive form overexpression (SvbAct) and SvbREP constitutive isoform overexpression (SvbRep). Three biological replicates were performed for each genotype, on different calendar days.