Nuclear simple sequence repeats primer pairs for Rhizoglyphus robini showing polymorphism across individuals.
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https://figshare.com/articles/Nuclear_simple_sequence_repeats_primer_pairs_for_Rhizoglyphus_robini_showing_polymorphism_across_individuals_/7665632/1
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In Stewart et al., 2019,"The role of genetic diversity in the evolution and maintenance of environmentally-cued, male alternative reproductive tactics", BMC Evolutionary Biology, we tested 16 nuclear simple sequence repeats (nSSRs) primer pairs designed for Rhizoglyphus robini, optimizing the primer pairs and concomitant PCR protocol for our own populations using Dreamtaq polymerase (Thermo Fisher Scientific). After protocol optimization, we found only 9 of the 16 nSSRs amplified well for our populations, of which 3 loci revealed fixation, and 6 demonstrating both clean/readable peaks and polymorphism across individuals. These 6 nSSRs were chosen for the genotyping of all remaining individuals. Here, the datasets generated and analysed in our study are presented.<br>
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figshare
创建时间：
2019-02-02
