Integrative assessment of sulfoxaflor effects on gene expression, reproduction, and behavior in the bumblebee <em>Bombus impatiens</em>

Social insect pollinators, such as bumblebees, face increasing threats from environmental agrochemicals; yet the sublethal effects of these compounds across different levels of biological organization remain poorly understood. This study uses an integrative approach to examine how chronic exposure to sublethal concentrations of the insecticide sulfoxaflor affects the common eastern bumblebee (Bombus impatiens). Microcolonies of worker bees were fed sulfoxaflor-treated sugar water for 21 days. We then assessed changes in molecular, physiological, and behavioral traits resulting from sulfoxaflor exposure. Transcriptomic analysis revealed extensive differential gene expression in ovaries, but not brains, of exposed bees. Bees exposed to sulfoxaflor showed upregulated cellular signaling pathways and downregulated genes associated with oogenesis and mitosis. Moreover, sulfoxaflor-exposed bees showed reduced tissue-biased gene expression, suggesting broader disruption in tissue-specific regul..., , , # Data from: Integrative assessment of sulfoxaflor effects on gene expression, reproduction, and behavior in the bumblebee *Bombus impatiens* [https://doi.org/10.5061/dryad.xsj3tx9rj](https://doi.org/10.5061/dryad.xsj3tx9rj) ## Description of the data and file structure ### Files and variables #### File: The_Chronic_Raw_Data.xlsx 1. Experimental Data; Columns represent experiment, Cage, # Bees, Bee Age, Treatment, Origin Colonies, Exposure Date, # Mortalities, # Bees final, Behavioral Assay, # Honey Pots, # Honey Pots/Bee, # Eggs, # Eggs/Bee, % Syrup Overall per bee 2. Ovary Mass Data; Columns represent SFX concentration mg/L, Cage, Individual, Ovary mass (mg) 3. Crawling Behavior Data; Columns represent Cage, treatment, Bee, average velocity (cm/min), % time spent moving, distance traveled (cm), frequency of head movements 4. Aggression Behavior Data; Columns represent treatment, cage, bee, trial, disturbance leg response (0, 1, or 2), Bite (y or n), sting (y or n), and buzz (y or..., ,