Oligos used in this study.
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https://figshare.com/articles/dataset/An_Integrated_System_for_Precise_Genome_Modification_in_Escherichia_coli_/1533876
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Oligos used for amplification and recombineering of the landing pad are designated with “LP”. Primers used for colony PCR verification are designated “ver”. Primers for amplifying the lac operon, mCherry, or ECFP with locus specific homology are designated as “lac”, “mCherry”, or “CFP” respectively. Finally, oligos used to complete the deletion of rrnB are designated “Pos” or “Neg”. (DOCX)
创建时间：
2015-12-03
