Figs

<b>Detection of IgG anti-<i>Leishmania</i> antigen by flow cytometry as a diagnostic test for cutaneous leishmaniasis</b><b>Figure legend</b><b>Figure 1. Gate strategy to determine beads population to be analyzed.</b> A gate was performed based on FSC and SSC and than mean florescence intensity was assessed based on FITC staining. <b>Figure 2. Antibody titers assessed by ELISA.</b> Anti-SLA IgG titers from healthy subjects (HS), cutaneous leishmaniasis patients (CL), Montenegro positive individuals (DTH+) and Chagas disease patients (CD), assessed by ELISA. Cut off was determined by a ROC curve containing absorbances from CL patients and healthy subjects. OD, optical density. <b>Figure 3. Antibody titers assessed by flow cytometry.</b> Anti-SLA IgG titers from healthy subjects (HS), cutaneous leishmaniasis patients (CL), Montenegro positive individuals (DTH+) and Chagas disease patients (CD), assessed by flow cytometry. Cut off was determined by a ROC curve containing MFI from CL patients and healthy subjects. MFI, mean fluorescence intensity.