RNA-Seq from two horse mint parents and two selected members of their F2 population

All mint lines were grown in a greenhouse with supplemental lighting in Pullman, WA, USA. Leaves were harvested before flowering. Secretory gland cells were immediately harvested by blending leaves in an extraction buffer and filtering over differently sized nylon meshes. Total RNA was isolated using the Qiagen RNeasy Plant Mini Kit (Qiagen, Germantown, MD, USA) with an on-column DNA digestion protocol and sent for sequencing. Quality assessment was done using a Bioanalyzer (Agilent, Santa Clara, CA, USA). Sequencing libraries were prepared and Illumina sequencing was performed by a third party vendor.