KSM fits for 320 bait-prey pairs of NUPs in KARMA assays

Supplementary analyzed data from Manuscript:
TITLE: Maturation kinetics of a multiprotein complex revealed by metabolic labeling.
JOURNAL: CELL.
Article Type: Research Article
Authors: Evgeny Onischenko*, Elad Noor*, Jonas S. Fischer*, Ludovic Gillet, Matthias Wojtynek, Pascal Vallotton, Karsten Weis
*Equally Contributing Authors
Corresponding Authors: Evgeny Onischenko and Karsten Weis

Related to Figures S4, Figure S5, and Table S3;

KSM fitting curves for 320 bait-prey pairs characterised in the KARMA assays.
Individual plots: The red line represents the expected labeling of the reference proteins as a function of time, and is used to find the effective sampling time for each sample (i.e. the x-value of each point). Then, we try to fit the 3-step KSM (purple line). Preys with metabolic labeling exceeding the reference protein were fitted with a 4-step model. 𝜙1: effective maturation pool, 𝜙2: accessible fraction of the mature state.