Nitrate reductase activity after functional complementation of an E. coli moeB− strain with the Mycobacterial proteins MoeZR, MoeBR, MoaD1, MoaD2, and CysO.

aMBP-MoeBR and MBP-MoeZR were coexpressed in an E. coli moeB− strain together with His6-MoaD1, His6-MoaD2, His6-MoaX or His6-CysO as indicated.bNitrate reductase activity was determined in crude cell extracts by a spectroscopic assay using benzyl viologen as described by Jones and Garland [27]. One unit of nitrate reductase activity is described as the production of 1 µmol of nitrite per min per O.D. 600. Assays were performed in triplicate.Significant differences at a level of Pt test) are indicated as **. All values represent means ± standard deviation (SD) for n = 9.csignificant difference of the MoeBR complemented strain compared to the E. coli moeB− strain.dsignificant differences of the MoeBR/MoaD1 and MoeBR/MoaD2 complemented strains to the MoeBR complemented E. coli strain.esignificant differences of the MoeZR/MoaD1 and MoeZR/MoaD2 complemented strains to the MoeZR complemented E. coli strain.