Bone Marrow Stroma Cells: Vehicle Control vs. Substance P Treated

Gene expression profiling by cDNA microarray of bone marrow stromal cells from three healthy donors treated as follows: vehicle control; or stimulated with 10 nM substance P. Hematopoiesis is tightly regulated by the bone marrow (BM) niche. The niche is robust, allowing for the return of hematopoietic homeostasis after insults such as infection. Hematopoiesis is partly regulated by soluble factors such as neuropeptides, substance P (SP) and neurokinin A (NK-A), which mediate hematopoietic stimulation and inhibition, respectively. The hematopoietic effects of SP and NK-A are mostly mediated via BM stroma. Array analyses with 2400 genes indicated distinct changes in SP-stimulated BM stroma. Bone marrow stromal cells were harvested from three healthy donors. Two-condition experiment comparing gene expression of control bone marrow stromal cells vs. substance P treated bone marrow stromal cells for each donor. Biological replicates: 3 vehicle treated, 3 SP treated.