Single-cell RNA sequencing of murine hearts for studying the development of the cardiac conduction system

The development of the cardiac conduction system (CCS) is essential for correct heart function. However, critical details on the cell types populating the CCS in the mammalian heart during the development remain to be resolved. Using single-cell RNA sequencing, we generated a large dataset of transcriptomes of ~0.5 million individual cells isolated from murine hearts at six successive developmental corresponding to the early, middle and late stages of heart development. The dataset provides a powerful library for studying the development of the heart's CCS and other cardiac components. Our initial analysis identified distinct cell types between 20 to 26 cell types across different stages, of which ten are involved in forming the CCS. Our dataset allows researchers to reuse the datasets for data mining and a wide range of analyses. Collectively, our data add valuable transcriptomic resources for further study cardiac development, such as gene expression, transcriptional regulation and functional gene activity in developing hearts, particularly the CCS. We isolated and collected the single cells from embryos or hearts at six developmental stages, including E8.5, E10.5, E12.5, E14.5, E16.5 and postnatal P3，two biological replicates per period using the standard enzymatic method.Single cells were prepared following 10× Genomics, Inc (Pleasanton, CA) protocol. Please note that processed data files generated from two replicates (i.e. E16_5_1_1_7*, p3_* samples) are linked to the first replicate records (i.e. E16_5_1_7, p3_1).