Cachectic parameters in control and treated mice at day 21.

In cancer cachectic groups (groups 3 and 4), Lewis Lung Carcinoma (LLC) cells transfected with IL-6 cDNR (LLC-IL6) were implanted subcutaneously into a total of 20 C57BL/6J mice. We administered MR16-1, anti-murine IL-6 receptor monoclonal antibody, intraperitoneally at a dose of 20 mg/kg once a week into 10 mice (Group 4), while 0.9% saline into 10 mice (Group 3), respectively. Eight mice in both the MR16-1 and control groups survived for 21 days after the tumor inoculation. After sacrifice, these mice were evaluated for cachectic parameters. Carcass weight was weight without tumor. Data represent mean ± S.D. Statistical significance was evaluated with Turkey’s test after a one-way ANOVA. *Significantly different from group 1 (tumor (−), MR16-1 (−)); p<0.01. **Significantly different from group 1 (tumor (−), MR16-1 (−); p<0.05. ***Significantly different from group 3 (tumor (+), MR16-1 (−)); p<0.01. ****Significantly different from group3 (tumor (+), MR16-1 (−)); p<0.05.