Raw sequence reads from CELL-SELEX experiment targeting CCR7 (9 rounds of selection)

Cell-based SELEX was performed using an RNA library containing a randomized 30-nt region flanked by fixed primer sequences. Cell-based selection was performed as previously described (Kim, Gu et al 2013), by employing open PCR for DNA amplification during each selection round. Positive selection was performed on Hela cells transduced with a bicistronic lentiviral vector expressing the target surface receptor and GFP, while unmodified Hela cells, which lack expression of the target receptor, were used for negative selection. High throughput sequencing (HTS) was performed on the positive selection at rounds 0, 1, 3, 5, 6, 7, 8, and 9.