Dissociation constants (K<sub>d</sub>) for the binding of thrombin to fibrin, factor Va and GpIbα269-286ppp.
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The binding of thrombin or FPR-thrombin to immobilized γA/γA-fibrin, γA/γ′-fibrin, factor Va or GpIbα269-286ppp was quantified using SPR. Kd values for thrombin binding to γA/γA-fibrin and GpIbα269-286ppp were determined by steady state analysis, whereas bivalent binding of thrombin to γA/γ′-fibrin and factor Va was determined by kinetic analysis for a two-site model using BIAevaluation software. Kd values are shown as mean ± S.D. for three separate experiments.
创建时间：
2016-06-21
