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H218O-RNA-DNA qSIP of soil microorganisms

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NIAID Data Ecosystem2026-03-10 收录
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The objective of this study is to elucidate rRNA and rDNA synthesis among soil microorganisms using a combined RNA and DNA quantitative stable isotope probing (qSIP) with isotopically labeled water. During incubation of soil with 18O-water, metabolically active or growing taxa incorporate the oxygen isotopes into newly synthesized nucleic acids. These taxa are subsequently identified by sequencing of the ultracentrifuged and fractionnated rRNA and rDNA.

创建时间:
2017-10-31
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