Loss of Ing4 enhances hematopoietic regeneration in multipotent progenitor cells

Gene expression of ING4-/- LT-HSCs, ST-HSCs, and MPPs were analyzed for gene expression at steady-state. Overall design: Bone marrow from 8-10 mice was harvested and pooled, and lineage depleted. 1,000-10,000 LT- HSCs, ST-HSCs, and MPPs were sorted separately into Buffer RLT Plus (Qiagen) for both WT or Ing4-/- mice. Total RNA was isolated with the RNeasy Micro column (QIAGEN). Library construction, sequencing by Illumina HiSeq, and initial bioinformatics analyses were conducted by GENEWIZ. Counts obtained were used to perform Gene Set Enrichment Analysis. Ing4-/- LT- and ST-HSCs were compared together against WT LT- and ST-HSCs or Ing4-/- LT-, ST-HSC and MPPs were compared together against WT.