Cucurbita maxima cultivar:2013-12 and 9-6 Raw sequence reads

A high-density genetic map can provide a basis for quantitative trait locus (QTL) mapping and marker-assisted selection (MAS) based on single-nucleotide polymorphisms (SNPs). Specific length amplified fragment sequencing (SLAF-seq) has been successfully applied to construct high-density maps of many species but not Cucurbita maxima. In this study, 100 F2 populations from two morphologically diverse parents were used to successfully construct a high-density genetic map of C. maxima using the SLAF-seq method. A total of 8,406 SNPs were used to construct a 3,376.87 cM genetic map for 20 linkage groups (LGs) with an average genetic distance of 0.47 cM.