Analytical sensitivity for the satDNA detection in GEA samples spiked with T. cruzi and Exo-IPC DNA.
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Assay was tested with genomic DNA extracted from GEA samples with different T. cruzi (Dm28c) concentrations, ranging from 10 to 0.01 T. cruzi equivalents/mL. All GEA supernatant samples were spiked with 2 μL EXO-IPC DNA prior DNA extraction. Results are shown as Ct mean ± SD for satDNA and Exo-IPC at the multiplex real time PCR assay.
创建时间:
2021-02-02



