scRNAseq of splenic T cells from Myc transgenic mice

Age-related thymic involution was prevented or reversed by overexpressing Myc either in a constitutive or inducible manner in thymic epithelial cells (TEC), respectively. To assess the impact of Myc overexpression in TEC on the peripheral T cell compartment scRNAseq was performed on splenic T cells isolated from middle-aged mice, that constitutively overexpress Myc, and aged mice that overexpress Myc in an inducible manner. Infection with certain T. gondii strains results in age-dependent mortality in mice. scRNAseq was performed to assess the impact of T. gondii infection on the peripheral T cell compartment in aged mice where thymic involution was reversed and wild type mice.