Anterior-Posterior gene expression differences in zebrafish Vagus motor neurons

We profiled the transcriptome of anterior and posterior zebrafish Vagus motor neurons at 28-30 hours post fertilization to identify genes that are differentially expressed between these two cell populations. We obtained cells by photoconverting the anterior or posterior 1/4 of Kaede-expressing Vagus motor neurons, dissecting the posterior hindbrain, dissociating to single cell suspension, and manually picking photoconverted cells. We sequenced 3 replicates of 100 cells per population.