Data from Species Distribution, Phylogenetic Structure, and Functional Roles of Detritius Inhabiting Fungi Across Contrasting Aquatic Environments.

This version 2 dataset contains 34 files in total with one (1) additional file, called "Culture-dependent Isolate table with taxonomic determination and sequence data.csv". The remaining files (33) are identical to version 1. The following is the information about the new file and its variables: <b>Culture-dependent Isolate table with taxonomic determination and sequence data.csv</b>: Culture table with assigned taxonomy from NCBI. Single direction sequence for each isolate is include if one could be obtained. Sequence is derived from ITS1F-ITS4 PCR amplicons, with Sanger sequencing in one direction using ITS5. The files contains 20 variables with explanation as below: IsolateNumber : unique number identify each isolate cultured Time: season in which the sample was collected Location: the specific name of the location Habitat: type of habitat : either stream or peatland State: state in the USA in which the specific location is located Incubation_pH ID: pH of the medium during isolation of fungal cultures Genus: phylogenetic genus of the fungal isolates (determined by sequence similarity) Sequence_quality: base call quality of the entire sequence used for blast analysis, if known %_coverage: sequence coverage reported from GenBank %_ID: sequence similarity reported from GenBank Life_style : ecological life style if known Phylum: phylogenetic phylum as indicated by Index Fungorum Subphylum: phylogenetic subphylum as indicated by Index Fungorum Class: phylogenetic class as indicated by Index Fungorum Subclass: phylogenetic subclass as indicated by Index Fungorum Order: phylogenetic order as indicated by Index Fungorum Family: phylogenetic Family as indicated by Index Fungorum ITS5_Sequence: single direction sequence used for sequence similarity match using blastn. Primer ITS5 Fasta: sequence with nomenclature in a fasta format for easy cut and paste into phylogenetic software Note: blank cells mean no data is available or unknown.

本版2.0数据集共计包含34个文件，其中新增一个文件，命名为“基于培养的分离物表：包含分类鉴定和序列数据.csv”。其余33个文件与版本1完全相同。以下为新增文件及其变量信息：<b>基于培养的分离物表：包含分类鉴定和序列数据.csv</b>：该文件包含来自NCBI的分类学分配的物种表。若可获得，每个分离物均包含一个方向的序列。序列由ITS1F-ITS4 PCR扩增子生成，并使用ITS5进行单方向Sanger测序。文件包含20个变量，具体说明如下：IsolateNumber：识别每个培养分离物的唯一编号Time：样本采集的季节Location：特定地点的名称Habitat：栖息地类型：河流或泥炭地State：美国特定地点所在的州Incubation_pH ID：真菌培养分离过程中培养基的pH值Genus：真菌分离物的系统发育属（通过序列相似性确定）Sequence_quality：用于BLAST分析整个序列的碱基调用质量，若已知%_coverage：从GenBank报告的序列覆盖率%_ID：从GenBank报告的序列相似度Life_style：若已知，生态生活方式Phylum：由Index Fungorum指示的系统发育门Subphylum：由Index Fungorum指示的系统发育亚门Class：由Index Fungorum指示的系统发育纲Subclass：由Index Fungorum指示的系统发育亚纲Order：由Index Fungorum指示的系统发育目Family：由Index Fungorum指示的系统发育科ITS5_Sequence：用于BLASTn序列相似度匹配的单方向序列Primer ITS5 Fasta：以fasta格式命名的序列，便于剪切粘贴至系统发育软件中备注：空白单元格表示无数据或未知。