Analysis of miRNA expression in human Oral epithelial cells induced by S. gordonii

The mechanisms through which oral commensal bacteria mitigates uncontrolled inflammatory responses in the oral mucosa remain unknown. Here we evaluated the ability of S. gordonii to stimulate the expression of miRNAs in oral epithelial cells with potential to target chemokine expression. The human oral epithelial cell line (OKF6) was exposed to different MOIs of S. gordonii for 24h and expression analysis of miRNAs performed using the Affymetrix platform. Oral epithelial cells (OKF6) were grown in 24-well plates overnight and exposed to different OEC:S. gordonii MOIs (1:10; 1:50; 1:100) for 24h, followed by RNA isolation and miRNA expression analysis using the GeneChip miRNA 4.0 Array.